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KMID : 0381120170390111205
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Genes and Genomics
2017 Volume.39 No. 11 p.1205 ~ p.1213
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The causal deletions in the second exon of An-3 closely associated with awn development and rice yield
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Liu Lilong
Li Yunzhen
Wei Minyi
Wu Zishuai
Liu Fang
Qiu Yongfu
Luo Jijing
Li Rongbai
Qin Baoxiang
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Abstract
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Awn is one of important traits during rice domestication. To understand the development of rice awn and the roles it played in rice domestication, we preliminary mapped a major QTL An-3 for awn development using chromosome segment substitution line CSSL138 developed by introgressed genomic fragments of long-awned Guangxi common wild rice (GXCWR, Oryza rufipogon Griff.) into genetic background of short-awned indica cultivar 93?11. An-3 was then fine mapped to a 7-kb region of chromosome 8. An epidermal patterning factor-like protein gene was identified as the single candidate gene corresponding to this QTL. An-3 was showed to be an allele of RAE2 and GAD1, and negatively regulated 1000-grains weight, grain length, and length?width ratio. Comparing with the coding sequences of An-3 from CSSL138, a 2- and 4-bp frame-shift deletions in the second exon were identified in 93?11 and Nipponbare, respectively. Taken together, our results provide valuable natural variation in the alleles of An-3 between common wild rice and cultivated rice, which will be helpful in clarifying the mechanism of awn development and promoting the application of an-3 in genetic improvement of rice yield traits.
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KEYWORD
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Rice, Awn, An-3, Epidermal patterning factor-like protein
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